• Attrezzatura generale (frigoriferi, congelatori, pHmetro, bilance, stufe, autoclave, bagno termostatico, agitatori magnetici)
• Apparato elettroforesi verticale 1D e Blotting
• Apparato elettroforesi orizzontale
• 2 Cappe a flusso laminare
• 2 Cappe chimiche
• Centrifughe da banco
• 2 Incubatori a CO2
• Microscopio Invertito
• Microscopio ottico
• Lettore elisa benchmark
• Termociclatore

Studio di:

• eventi trasduzionali evocati in risposta a chemioterapici a base di platino in cellule sane e tumorali di mammifero.
• funzioni biologiche degli ormoni estro-progestinici e dei loro recettori negli epiteli mammario, laringeo ed endometriale e nei loro carcinomi.
• funzioni biologiche dell'Angiotensina II, della Bradichinina e dell’ATP/UTP, in particolare quelle legate all'omeostasi del sodio, in sistemi cellulari animali e fisiopatologici umani
• meccanismi della comunicazione cellulare mediata da ormoni.
• meccanismi di trasmissione e trasduzione in cellule animali ed umane
• Stato di sviluppo antropometrico e condizionale, del monitoraggio del carico d’allenamento e dell’intervento nutrizionale su atleti professionisti

Attività di ricerca e di formazione con la messa a punto di tecniche sperimentali per lo studio di funzioni fisiologiche in cellule umane ed animali

• Responsabile Scientifico: Marsigliante
• Santo Danieli Antonio (personale tecnico categoria C, 10%)
• Antonella Muscella (P.A. MEDF/01)
• Giulia My (dottoranda XXXVI ciclo)
• Luca Giulio Cossa (assegnista di ricerca)

Nel corso degli anni sono state realizzate numerose collaborazioni, che hanno prodotto svariate pubblicazioni scientifiche, con:

• G.P. Vinson & J.P. Hinson - St. Bartholomew’s Hospital, Università di Londra
• S.M. Laird, Sheffield City Polytechnic, Università di Sheffield
• E. Jimenez, Università di Malaga
• A.W. Goode, Medical College di Londra
• A. Pellissier, Università di Grenoble, Francia
• M. Paulmichl, Università di Salzburg, Austria
• L. Resta, Università di Bari
• A. Paradiso, M. Correale, IRCCS di Bari
• F. Aloisi, G. Levi, ISS, Roma
• L. Ulianich, CNR, Napoli
• C. Vetrugno, IRCCS San Raffaele, Milano
• P. Salvatore, C. Manca, A.O. ‘‘V Fazzi’’, Lecce
• A. Cimmino, Università di Bari
• M. Mallardo, L. Annunziato, Università Napoli Federico II
• P. Vito, Università del Sannio, Benevento
• A. Leone, CNR, Lecce
• P. Beck-Peccoz, Università di Milano
• F. Biagioni e M.T. Calierno, IRCCS di Pozzilli (IS)
• F. Fornai, Università Pisa

ll laboratorio di Fisiologia Cellulare è coinvolto nel tutoraggio di studenti impegnati nello svolgimento della tesi di laurea, dottorandi di ricerca, contrattisti e assegnisti di ricerca interessati all’approfondimento delle tematiche di ricerca del laboratorio.

Il laboratorio di Fisiologia cellulare è stato utilizzato da studenti dei corsi di dottorato in “Biologia e Biotecnologie” e in “Scienze e Tecnologie Biologiche e Ambientali" per svolgere le loro attività di Tesi di dottorato di ricerca (10 Tesi di dottorato).

Inoltre, il laboratorio di Fisiologia cellulare è stato utilizzato da studenti dei corsi di laurea in Scienze Biologiche e in Biotecnologie per svolgere le loro attività di Tesi sperimentali triennali e magistrali (70 Tesi sperimentali)

• PNR 2015-2020, area di specializzazione “Salute” - “Sviluppo di biomarcatori diagnostici per la medicina di precisione e la terapia personalizzata” FISH RISE - ARS01_01053
• FEAMP 2014/2020 “Progetto per lo sviluppo di siti e infrastrutture legati all’acquacoltura pugliese e per la riduzione degli impatti ambientali degli interventi”
• PRIN – 2009 “Sintesi, caratterizzazione e studio del pathway d'induzione apoptotica di composti antitumorali di Pt per target non genomici”
• PRIN – 2004 “Valutazione dell'attivita' biologica di nuove molecole analoghe del cisplatino conformazionalmente stabilizzate”
• PRIN - 2003 “Il controllo extracellulare dell'espressione e dell'attivit. della pendrina in linee cellulari tiroidee”
• PRIN – 2001 “Trasporto di ioni e acqua in cellule tiroidee: caratterizzazione molecolare, regolazioni e rapporti con la trasformazione neoplastica”
• PON - ‘Rigenerazione di tessuti nervosi ed osteocartilaginei mediante innovativi approcci di tissue engineering’ (RINOVATIS)
• CNR - “Messa a punto e caratterizzazione di cellule endoteliali umane in coltura (HUVEC) quale modello di studio di processi fisiopatologici vascolari”
• POR - “BIO-LAT: Un metodo di validazione biologica della bioattività di componenti attivi del latte da applicare per nuovi prodotti lattiero caseari di alto valore funzionale e nutraceutico”

• Marzouki H, Soussi B, Selmi O, Hajji Y, Marsigliante S, Bouhlel E, Muscella A, Weiss K, Knechtle B. Effects of Aquatic Training in Children with Autism Spectrum Disorder Biology 2022, 11, 657. https://doi.org/10.3390/biology11050657
• My G, Marsigliante S, Bianco A, Zangla D, da Silva CM, Muscella A. Biological, Psychological, and Physical Performance Variations in Football Players during the COVID-19 Lockdown: A Prospective Cohort Study Int J Environ Res Pub Health 2022 19(5) 2022 Article number 2739
• Di Maglie A, Marsigliante S, My G, Colazzo S, Muscella A. Effects of a physical activity intervention on schoolchildren fitness Physiological Reports 2022 -Open Access- Volume 10, Issue 2, Article number e15115
• Muscella A, Stefàno E, De Bellis L, Nutricati E, Negro C and Marsigliante S. Antitumor and antimigration effects of Salvia clandestina L. extract on osteosarcoma cells Ann. N.Y. Acad. Sci. 2021, 1–14 - doi: 10.1111/nyas.14601
• Muscella A, Stefàno E, Calabriso N, De Pascali SA, Fanizzi FP, Marsigliante S Role of epidermal growth factor receptor signaling in a Pt(II)-resistant human breast cancer cell line Biochemical Pharmacology 2021, 192, 114702.
• Trapani A, Corbo F, Agrimi G, Ditaranto N, Cioffi N, Perna F, Quivelli A, Stefàno E, Lunetti P, Muscella A, Marsigliante S, Cricenti A, Luce M, Mormile C, Cataldo A and Stefano Bellucci Oxidized Alginate Dopamine Conjugate: In Vitro Characterization for Nose-to-Brain Delivery Application Materials 2021, 14, 3495. https://doi.org/10.3390/ma14133495
• Muscella A, Stefàno E, Marsigliante S. The effects of training on hormonal concentrations and physical performance of football referees. Physiological Reports 2021 -Open Access- Volume 9, Issue 8, Article number e14740
• De Castro F, Stefàno E, Migoni D, Iaconisi GN, Muscella A, Marsigliante S, Benedetti M, Fanizzi FP. Synthesis and evaluation of the cytotoxic activity of water-soluble cationic organometallic complexes of the type [Pt(η1-C2H4OMe)(L)(phen)]+ (L = NH3, DMSO; phen = 1,10-phenanthroline) Pharmaceutics 2021 -Open Access- Volume 13, Issue 5, Article number 642
• Muscella A, Stefàno E, Di Maglie A, Marsigliante S. Referees' physical performance over a soccer season. Sport Sciences for Health, 2020, 16(4), pp. 765–773
• Muscella A, Stefàno E, Marsigliante S. The effects of exercise training on lipid metabolism and coronary heart disease. Am J Physiol Heart Circ Physiol. 2020 Jul 1;319(1):H76-H88. doi: 10.1152/ajpheart.00708.2019.
• Muscella A, Stefàno E, Lunetti P, Capobianco L, Marsigliante S. The regulation of fat metabolism during aerobic exercise. Biomolecules. 2020 Dec 21;10(12):1699. doi: 10.3390/biom10121699.
• Muscella A, Cossa LG, Vetrugno C, Marsigliante S. Bradykinin stimulates prostaglandin E2 release in human skeletal muscular fibroblasts. Mol Cell Endocrinol. 2020 May 1;507:110771. doi: 10.1016/j.mce.2020.110771.
• Muscella A, Vetrugno C, Cossa LG, Marsigliante S. TGF-β1 activates RSC96 Schwann cells migration and invasion through MMP-2 and MMP-9 activities. J. Neurochem. 2020; 153(4):525-538. doi: 10.1111/jnc.14913
• Stefàno E, Marsigliante S, Vetrugno C, Muscella A. Is mitochondrial DNA profiling predictive for athletic performance? Mitochondrion. 2019 Jul;47:125-138. doi: 10.1016/j.mito.2019.06.004. Review.
• Muscella A, Vetrugno C, Spedicato M, Stefàno E, Marsigliante S. The effects of training on hormonal concentrations in young soccer players. J Cell Physiol. 2019 Apr 16. doi: 10.1002/jcp.28673.
• Antonaci G, Cossa LG, Muscella A, Vetrugno C, De Pascali SA, Fanizzi FP, Marsigliante S. [Pt(O,O'-acac)(γ-acac)(DMS)] Induces Autophagy in Caki-1 Renal Cancer Cells. Biomolecules. 2019 Mar 6;9(3). pii: E92. doi: 10.3390/biom9030092.
• De Castro F, Benedetti M, Antonaci G, Del Coco L, De Pascali SA, Muscella A, Marsigliante S, Fanizzi FP. Response of Cisplatin Resistant Skov-3 Cells to [Pt(O,O'-Acac)(γ-Acac)(DMS)] Treatment Revealed by a Metabolomic ¹H-NMR Study. Molecules. 2018 Sep 9;23(9). pii: E2301. doi: 10.3390/molecules23092301.
• Muscella A, Cossa LG, Vetrugno C, Antonaci G, Marsigliante S. ADP sensitizes ZL55 cells to the activity of cisplatin. J Cell Physiol. 2018 Aug 25. doi: 10.1002/jcp.27224.
• Muscella A, Cossa LG, Vetrugno C, Antonaci G, Marsigliante S. Adenosine diphosphate regulates MMP2 and MMP9 activity in malignant mesothelioma cells. Ann N Y Acad Sci. 2018 Nov;1431(1):72-84. doi: 10.1111/nyas.13922.
• Muscella A, Cossa LG, Vetrugno C, Antonaci G, Marsigliante S. Inhibition of ZL55 cell proliferation by ADP via PKC-dependent signalling pathway. J Cell Physiol. 2018 233(3):2526-2536. doi: 10.1002/jcp.26128.
• Muscella A, Vetrugno C, Cossa LG, Antonaci G, Barca A, De Pascali SA, Fanizzi FP, Marsigliante S. Apoptosis by [Pt(O,O'-acac)(γ-acac)(DMS)] requires PKC-δ mediated p53 activation in malignant pleural mesothelioma. PLoS One. 2017 Jul 12;12(7):e0181114. doi: 10.1371/journal.pone.0181114. eCollection 2017.
• Muscella A, Vetrugno C, Marsigliante S. CCL20 promotes migration and invasiveness of human cancerous breast epithelial cells in primary culture. Mol Carcinog. 2017 Nov;56(11):2461-2473. doi: 10.1002/mc.22693.
• Muscella A, Vetrugno C, Cossa LG, Antonaci G, De Nuccio F, De Pascali SA, Fanizzi FP, Marsigliante S. In Vitro and In Vivo Antitumor Activity of [Pt(O,O'-acac)(γ-acac)(DMS)] in Malignant Pleural Mesothelioma. PLoS One. 2016 Nov 2;11(11):e0165154. doi: 10.1371/journal.pone.0165154.
• Muscella A, Vetrugno C, Biagioni F, Calabriso N, Calierno MT, Fornai F, De Pascali SA, Marsigliante S, Fanizzi FP. Antitumour and antiangiogenic activities of [Pt(O,O'-acac)(γ-acac)(DMS)] in a xenograft model of human renal cell carcinoma. Br J Pharmacol. 2016 Sep;173(17):2633-44. doi: 10.1111/bph.13543.
• Marsigliante S, Vetrugno C, Muscella A. Paracrine CCL20 loop induces epithelial-mesenchymal transition in breast epithelial cells. Mol Carcinog. 2016 Jul;55(7):1175-86. doi: 10.1002/mc.22360.
• Muscella A, Vetrugno C, Antonaci G, Cossa LG, Marsigliante S. PKC-δ/PKC-α activity balance regulates the lethal effects of cisplatin. Biochem Pharmacol. 2015 Nov 1;98(1):29-40. doi: 10.1016/j.bcp.2015.08.103.
• D'Urso PI, D'Urso OF, Gianfreda CD, Mezzolla V, Storelli C, Marsigliante S. miR-15b and miR-21 as Circulating Biomarkers for Diagnosis of Glioma. Current Genomics 2015, Vol. 16 Issue: 5 Pages: 304-311
• Muscella A, Vetrugno C, Calabriso N, Cossa LG, De Pascali SA, Fanizzi FP, Marsigliante S. [Pt(O,O'-acac)(γ-acac)(DMS)] alters SH-SY5Y cell migration and invasion by the inhibition of Na+/H+ exchanger isoform 1 occurring through a PKC-ε/ERK/mTOR Pathway. PLoS One. 2014 Nov 5;9(11):e112186. doi: 10.1371/journal.pone.0112186. eCollection 2014. 101: De Pascali SA; Muscella A; Vetrugno C; Marsigliante S; Fanizzi FP. Synthesis, characterization and cytotoxicity of novel Pt(II) kappa O-2,O '-acetylacetonate complexes with nitrogen ligands. Inorg. Chim. Acta 2014, 412 : 88-93 doi: 10.1016/j.ica.2013.12.018
• Vetrugno C, Muscella A, Fanizzi FP, Cossa LG, Migoni D, De Pascali SA, Marsigliante S. Different apoptotic effects of [Pt(O,O'-acac)(γ-acac)(DMS)] and cisplatin on normal and cancerous human epithelial breast cells in primary culture. Br J Pharmacol. 2014 Nov;171(22):5139-53. doi: 10.1111/bph.12831. Epub 2014 Sep 5.
• Muscella A, Vetrugno C, Migoni D, Biagioni F, Fanizzi FP, Fornai F, De Pascali SA, Marsigliante S. Antitumor activity of [Pt(O,O'-acac)(γ-acac)(DMS)] in mouse xenograft model of breast cancer. Cell Death Dis. 2014 Jan 23;5:e1014. doi: 10.1038/cddis.2013.554.
• Muscella A, Vetrugno C, Fanizzi FP, Manca C, De Pascali SA, Marsigliante S. A new platinum(II) compound anticancer drug candidate with selective cytotoxicity for breast cancer cells. Cell Death Dis. 2013 Sep 12;4:e796. doi: 10.1038/cddis.2013.315.
• Rochira A, Damiano F, Marsigliante S, Gnoni GV, Siculella L. 3’,5’-Diiodo-l-thyronine induces SREBP-1 proteolytic cleavage block and apoptosis in human hepatoma (Hepg2) cells. Biochim Biophys Acta. 2013 Dec;1831(12):1679-89.
• Marsigliante S, Vetrugno C, Muscella A. CCL20 induces migration and proliferation on breast epithelial cells. J Cell Physiol. 2013 Sep;228(9):1873-83.
• D'Urso PI, D'Urso OF, Storelli C, Mallardo M, Gianfreda CD, Montinaro A, Cimmino A, Pietro C, Marsigliante S. miR-155 is up-regulated in primary and secondary glioblastoma and promotes tumour growth by inhibiting GABA receptors. Int J Oncol. 2012 Jul;41(1):228-34. doi: 10.3892/ijo.2012.1420. Epub 2012 Mar 28.
• Gnoni GV, Rochira A, Leone A, Damiano F, Marsigliante S, Siculella L. 3,5,3'triiodo-L-thyronine induces SREBP-1 expression by non-genomic actions in human Hep G2 cells J Cell Physiol. 2012 Jun;227(6):2388-97. doi: 10.1002/jcp.22974.
• D'Urso PI, D'Urso OF, Storelli C, Catapano G, Gianfreda CD, Montinaro A, Muscella A, Marsigliante S. Retrospective protein expression and epigenetic inactivation studies of CDH1 in patients affected by low-grade glioma. J Neurooncol. 2011 Aug;104(1):113-8.
• Muscella A, Calabriso N, Vetrugno C, Fanizzi FP, De Pascali SA, Marsigliante S. The signalling axis mediating neuronal apoptosis in response to [Pt(O,O'-acac)(-acac)(DMS)]. Biochem Pharmacol. 2011 Jun 1;81(11):1271-85. doi: 10.1016/j.bcp.2011.03.007.
• Muscella A, Calabriso N, Vetrugno C, Fanizzi FP, De Pascali SA, Storelli C, Marsigliante S. The platinum (II) complex [Pt(O,O'-acac)(gamma-acac)(DMS)] alters the intracellular calcium homeostasis in MCF-7 breast cancer cells. Biochem Pharmacol. 2011 Jan 1;81(1):91-103. Epub 2010 Sep 18. PubMed PMID: 20854797.
• Muscella A, Calabriso N, Vetrugno C, Urso L, Fanizzi FP, De Pascali SA, Marsigliante S. Sublethal concentrations of the platinum(II) complex [Pt(O,O'-acac)(gamma-acac)(DMS)] alter the motility and induce anoikis in MCF-7 cells. Br J Pharmacol. 2010 Jul;160(6):1362-77. PubMed PMID: 20590627; PubMed Central PMCID: PMC2938808.
• Urso L, Muscella A, Calabriso N, Vetrugno C, Jimènez E, Montiel M, Marsigliante S. Effects of cisplatin on matrix metalloproteinase-2 in transformed thyroid cells. Biochem Pharmacol. 2010 Mar 15;79(6):810-6.
• D'Urso OF, D'Urso PI, Storelli C, Marsigliante S. Solid phase subtractive cloning in differentially expressed genes identification. Mol Biol Rep. 2010 Mar;37(3):1435-8.
• Poltronieri P, Vetrugno C, Muscella A, Marsigliante S. Lactoferrin as an added-value whey component and a healthy additive in nutraceutical drinks Milk Consumption and Health, 2009, pp. 163–174 – Book chapter - Nova Science Publishers, Inc. - ISBN: 978-161728540-0;978-160741459-9
• D'Urso OF, D'Urso PI, Marsigliante S, Storelli C, Luzi G, Gianfreda CD, Montinaro A, Distante A, Ciappetta P. Correlative analysis of gene expression profile and prognosis in patients with gliomatosis cerebri. Cancer. 2009 Aug 15;115(16):3749-57. PubMed PMID: 19517475.
• D'Urso PI, D'Urso OF, Marsigliante S, Storelli C, Distante A, Sanguedolce F, Cimmino A, Luzi G, Gianfreda CD, Montinaro A, Ciappetta P. Gliomatosis cerebri type II: two case reports. J Med Case Reports. 2009 Jun 15;3:7225.
• D'Urso OF, Poltronieri P, Marsigliante S, Storelli C, Hernàndez M, Rodriguez-Làzaro D. A filtration-based real-time PCR method for the quantitative detection of viable Salmonella enterica and Listeria monocytogenes in food samples. Food Microbiol. 2009 May;26(3):311-6.
• Muscella A, Urso L, Calabriso N, Vetrugno C, Fanizzi FP, Storelli C, Marsigliante S. Functions of epidermal growth factor receptor in cisplatin response of thyroid cells. Biochem Pharmacol. 2009 Mar 15;77(6):979-92.
• Muscella A, Urso L, Calabriso N, Vetrugno C, Rochira A, Storelli C, Marsigliante S. Anti-apoptotic effects of protein kinase C-delta and c-fos in cisplatin-treated thyroid cells. Br J Pharmacol. 2009 Mar;156(5):751-63.
• Montiel M, Urso L, de la Blanca EP, Marsigliante S, Jimènez E. Cisplatin reduces endothelial cell migration via regulation of type 2-matrix metalloproteinase activity. Cell Physiol Biochem. 2009 23(4-6):441-8.
• De Pascali SA, Migoni D, Papadia P, Romano A, Marsigliante S, Pellissier A, Chardon-Noblat S, Ciccarese A, Fanizzi FP. New mononuclear and homodinuclear Pt(ii) complexes with heterocyclic nitrogen chelates: synthesis, characterization, intercalating ability and in vitro cytotoxic activity evaluation. Dalton Trans. 2008 Nov 21;(43):5911-21.
• Muscella A, Marsigliante S, Verri T, Urso L, Dimitri C, Bottà G, Paulmichl M, Beck-Peccoz P, Fugazzola L, Storelli C. PKC-epsilon-dependent cytosol-to-membrane translocation of pendrin in rat thyroid PC Cl3 cells. J Cell Physiol. 2008 Oct;217(1):103-12.
• Muscella A, Calabriso N, Fanizzi FP, De Pascali SA, Urso L, Ciccarese A, Migoni D, Marsigliante S. [Pt(O,O'-acac)(-acac)(DMS)], a new Pt compound exerting fast cytotoxicity in MCF-7 breast cancer cells via the mitochondrial apoptotic pathway. Br J Pharmacol. 2008 Jan;153(1):34-49.
• Vecchio VM, Benedetti M, Migoni D, De Pascali SA, Ciccarese A, Marsigliante S, Capitelli F, Fanizzi FP. Highly selective metal mediated ortho-alkylation of phenol. First platinum containing organometallic chromane analogues. Dalton Trans. 2007 Dec 28;(48):5720-5725
• Tinelli A, Vergara D, Leo G, Malvasi A, Casciaro S, Leo E, Montinari MR, Maffia M, Marsigliante S, Lorusso V. Human papillomavirus genital infection in modern gynecology: Genetic and genomic aspects Eur Clinics Obstet Gynaecol 2007 3, 1–6. https://doi.org/10.1007/s11296-007-0064-y
• Tinelli A, Vergara D, Martignago R, Leo G, Malvasi A, Tinelli R, Marsigliante S, Maffia M, Lorusso V. Ovarian cancer biomarkers: a focus on genomic and proteomic findings. Curr Genomics. 2007 Aug;8(5):335-342.
• Muscella A, Calabriso N, De Pascali SA, Urso L, Ciccarese A, Fanizzi FP, Migoni D, Marsigliante S. New platinum(II) complexes containing both an O,O'-chelated acetylacetonate ligand and a sulfur ligand in the platinum coordination sphere induce apoptosis in HeLa cervical carcinoma cells. Biochem Pharmacol. 2007 Jun 30;74(1):28-40.
• Romano S, Muscella A, Storelli C, Marsigliante S. Angiotensin II does not stimulate proliferation of rat thyroid PC Cl3 cell line. J Endocrinol. 2006 Dec;191(3):727-735.
• De Pascali SA, Migoni D, Papadia P, Muscella A, Marsigliante S, Ciccarese A, Fanizzi FP. New water-soluble platinum(II) phenanthroline complexes tested as cisplatin analogues: First-time comparison of cytotoxic activity between analogous four- and five-coordinate species. Dalton Trans. 2006 Nov 14;(42):5077-87.
• Ulianich L, Elia MG, Treglia AS, Muscella A, Di Jeso B, Storelli C, Marsigliante S. The sarcoplasmic-endoplasmic reticulum Ca2+ ATPase 2b regulates the Ca2+ transients elicited by P2Y2 activation in PC Cl3 thyroid cells. J Endocrinol. 2006 Sep;190(3):641-9.
• Greco S, Storelli C, Marsigliante S. Protein kinase C (PKC)-delta/-epsilon mediate the PKC/Akt-dependent phosphorylation of extracellular signal-regulated kinases 1 and 2 in MCF-7 cells stimulated by bradykinin. J Endocrinol. 2006 Jan;188(1):79-89.
• Muscella A, Urso L, Calabriso N, Ciccarese A, Migoni D, Fanizzi FP, Di Jeso B, Storelli C, Marsigliante S. Differential response of normal, dedifferentiated and transformed thyroid cell lines to cisplatin treatment. Biochem Pharmacol. 2005 Dec 19;71(1-2):50-60. Epub 2005 Nov 16. PubMed PMID: 16297374.
• Urso L, Muscella A, Calabriso N, Ciccarese A, Fanizzi FP, Migoni D, Di Jeso B, Storelli C, Marsigliante S. Differential functions of PKC-delta and PKC-zeta in cisplatin response of normal and transformed thyroid cells. Biochem Biophys Res Commun. 2005 Nov 11;337(1):297-305. PubMed PMID: 16182242.
• Muscella A, Storelli C, Marsigliante S. Atypical PKC-zeta and PKC-iota mediate opposing effects on MCF-7 Na+/K+ATPase activity. J Cell Physiol. 2005 Nov;205(2):278-85. PubMed PMID: 15887250.
• Greco S, Elia MG, Muscella A, Romano S, Storelli C, Marsigliante S. Bradykinin stimulates cell proliferation through an extracellular-regulated kinase 1 and 2-dependent mechanism in breast cancer cells in primary culture. J Endocrinol. 2005 Aug;186(2):291-301. PubMed PMID: 16079255.
• Elia MG, Muscella A, Romano S, Greco S, Di Jeso B, Verri T, Storelli C, Marsigliante S. Effects of extracellular nucleotides in the thyroid: P2Y2 receptor-mediated ERK1/2 activation and c-Fos induction in PC Cl3 cells. Cell Signal. 2005 Jun;17(6):739-49. Epub 2004 Nov 23. PubMed PMID: 15722198.
• Verri T, Dimitri C, Treglia S, Storelli F, De Micheli S, Ulianich L, Vito P, Marsigliante S, Storelli C, Di Jeso B. Multiple pathways for cationic amino acid transport in rat thyroid epithelial cell line PC Cl3. Am J Physiol Cell Physiol. 2005 Feb;288(2):C290-303. Epub 2004 Oct 13. PubMed PMID: 15483229.
• Greco S, Muscella A, Elia MG, Romano S, Storelli C, Marsigliante S. Mitogenic signalling by B2 bradykinin receptor in epithelial breast cells. J Cell Physiol. 2004 Oct;201(1):84-96. PubMed PMID: 15281091.
• Muscella A, Greco S, Elia MG, Storelli C, Marsigliante S. Differential signalling of purinoceptors in HeLa cells through the extracellular signal-regulated kinase and protein kinase C pathways. J Cell Physiol. 2004 Sep;200(3):428-39. PubMed PMID: 15254971.
• Ulianich L, Secondo A, De Micheli S, Treglia S, Pacifico F, Liguoro D, Moscato F, Marsigliante S, Annunziato L, Formisano S, Consiglio E, Di Jeso B. TSH/cAMP up-regulate sarco/endoplasmic reticulum Ca2+-ATPases expression and activity in PC Cl3 thyroid cells. Eur J Endocrinol. 2004 Jun;150(6):851-61.
• Zilli L, Schiavone R, Ingrosso L, Marsigliante S, Zonno V, Storelli C, Vilella S. Calcium channels are present in the apical plasma membranes of the hepatopancreatic B-cells of Marsupenaeus japonicus. J Comp Physiol B. 2003 Nov;173(8):661-7.
• Muscella A, Greco S, Elia MG, Storelli C, Marsigliante S. PKC-zeta is required for angiotensin II-induced activation of ERK and synthesis of C-FOS in MCF-7 cells. J Cell Physiol. 2003 Oct;197(1):61-8. PubMed PMID: 12942541.
• Greco S, Muscella A, Elia MG, Salvatore P, Storelli C, Mazzotta A, Manca C, Marsigliante S. Angiotensin II activates extracellular signal regulated kinases via protein kinase C and epidermal growth factor receptor in breast cancer cells. J Cell Physiol. 2003 Aug;196(2):370-7. PubMed PMID: 12811831.
• Muscella A, Elia MG, Greco S, Storelli C, Marsigliante S. Activation of P2Y2 receptor induces c-FOS protein through a pathway involving mitogen-activated protein kinases and phosphoinositide 3-kinases in HeLa cells. J Cell Physiol. 2003 May;195(2):234-40. PubMed PMID: 12652650.
• Marsigliante S, Muscella A, Elia MG, Greco S, Storelli C. Angiotensin II AT1 receptor stimulates Na+ -K+ATPase activity through a pathway involving PKC-zeta in rat thyroid cells. J Physiol. 2003 Jan 15;546(Pt 2):461-70
• Elia MG, Muscella A, Greco S, Vilella S, Storelli C, Marsigliante S. Disturbances in purinergic [Ca2+]i signaling pathways in a transformed rat thyroid cell line. Cell Calcium. 2003 Jan;33(1):59-68.
• Vilella S, Schiavone R, Zilli L, Marsigliante S, Storelli C. Characterization of Na(+)/H(+) antiporter activity in PC-Cl3 thyroid cells. Cell Physiol Biochem. 2003 13(4):207-214.
• Muscella A, Elia MG, Greco S, Storelli C, Marsigliante S. Activation of P2Y2 purinoceptor inhibits the activity of the Na+/K+-ATPase in HeLa cells. Cell Signal. 2003 15(1):115-21.
• Greco S, Muscella A, Elia MG, Salvatore P, Storelli C, Marsigliante S. Activation of angiotensin II type I receptor promotes protein kinase C translocation and cell proliferation in human cultured breast epithelial cells. J Endocrinol. 2002 Aug;174(2):205-14.
• Greco S, Elia MG, Muscella A, Storelli C, Marsigliante S. AT1 angiotensin II receptor mediates intracellular calcium mobilization in normal and cancerous breast cells in primary culture. Cell Calcium. 2002 Jul;32(1):1-10.
• Muscella A, Greco S, Elia MG, Jimènez E, Storelli C, Marsigliante S. Muscarinic acetylcholine receptor activation induces Ca2+ mobilization and Na+/K+-ATPase activity inhibition in eel enterocytes. J Endocrinol. 2002 May;173(2):325-34.
• Muscella A, Greco S, Elia MG, Storelli C, Marsigliante S. Angiotensin II stimulation of Na+/K+ATPase activity and cell growth by calcium-independent pathway in MCF-7 breast cancer cells. J Endocrinol. 2002 May;173(2):315-323.
• Marsigliante S, Elia MG, Di Jeso B, Greco S, Muscella A, Storelli C. Increase of [Ca(2+)](i) via activation of ATP receptors in PC-Cl3 rat thyroid cell line. Cell Signal. 2002 Jan;14(1):61-67.
• Montiel M, Pavìa J, Marsigliante S, Jimènez E. Activation of muscarinic acetylcholine receptors induces Ca(2+) mobilization in FRT cells. Cell Signal. 2001 Mar;13(3):207-212.
• Marsigliante S, Muscella A, Greco S, Elia MG, Vilella S, Storelli C. Na+/K+ATPase activity inhibition and isoform-specific translocation of protein kinase C following angiotensin II administration in isolated eel enterocytes. J Endocrinol. 2001 Feb;168(2):339-346.
• Greco S, Marsigliante S, Leo G, Storelli C. Co-expression of thymidine kinase and cathepsin D in 200 primary breast carcinomas. Cancer Lett. 2000 Nov10;160(1):13-19.
• Ingrosso L, Marsigliante S, Zonno V, Storelli C, Vilella S. An L-proline-dependent proton flux is located at the apical membrane level of the eel enterocytes. Am J Physiol Regul Integr Comp Physiol. 2000 Nov;279(5):R1619-R1624.
• Marsigliante S, Barker S, Jimenez E, Storelli C. Glucocorticoid receptors in the euryhaline teleost Anguilla anguilla. Mol Cell Endocrinol. 2000 Apr 25;162(1-2):193-201.
• Marsigliante S, Muscella A, Barker S, Storelli C. Angiotensin II modulates the activity of the Na+/K+ATPase in eel kidney. J Endocrinol. 2000 Apr;165(1):147-56.
• Marsigliante S, Muscella A, Vilella S, Storelli C. Dexamethasone modulates the activity of the eel branchial Na+/K+ATPase in both chloride and pavement cells. Life Sci. 2000 Mar 24;66(18):1663-1673.
• Muscella A, Aloisi F, Marsigliante S, Levi G. Angiotensin II modulates the activity of Na+,K+-ATPase in cultured rat astrocytes via the AT1 receptor and protein kinase C-delta activation. J Neurochem. 2000 Mar;74(3):1325-1331.
• Dimonte M, Leo G, Marsigliante S, Manca C. The prognostic significance of 99mTc-MDP breast scintigraphy. A comparison with histological and molecular parameters. Radiol Med. 1999 Nov;98(5):352-355.
• Marsigliante S, Biscozzo L, Leo G, Storelli C. Biphasic Scatchard plots of oestrogen receptors are associated with low pS2 levels in human breast cancers. Cancer Lett. 1999 Sep 20;144(1):17-23.
• Jimènez E, Caro MC, Marsigliante S, Montiel M. Angiotensin II receptor internalization and signaling in isolated rat hepatocytes. Biochem Pharmacol. 1999 May 15;57(10):1125-1131.
• Marsigliante S, Biscozzo L, Marra A, Nicolardi G, Leo G, Lobreglio GB, Storelli C. Computerised counting of tumour infiltrating lymphocytes in 90 breast cancer specimens. Cancer Lett. 1999 May 3;139(1):33-41.
• Muscella A, Marsigliante S, Vilella S, Jimenez E, Storelli C. Angiotensin II stimulates the Na+/H+ exchanger in human umbilical vein endothelial cells via AT1 receptor. Life Sci. 1999 65(22):2385-2394.
• Perniola R, Tamborrino G, Marsigliante S, De Rinaldis C. Assessment of enamel hypoplasia in autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). J Oral Pathol Med. 1998 Jul;27(6):278-282.
• Muscella A, Marsigliante S, Carluccio MA, Vinson GP, Storelli C. Angiotensin II AT1 receptors and Na+/K+ ATPase in human umbilical vein endothelial cells. J Endocrinol. 1997 Dec;155(3):587-593.
• Marsigliante S, Acierno R, Maffia M, Muscella A, Vinson GP, Storelli C. Immunolocalisation of angiotensin II receptors in icefish (Chionodraco hamatus) tissues. J Endocrinol. 1997 Aug;154(2):193-200.
• Marsigliante S, Muscella A, Vinson GP, Storelli C. Angiotensin II receptors in the gill of sea water- and freshwater-adapted eel. J Mol Endocrinol. 1997 Feb;18(1):67-76. PubMed PMID: 9061608.
• Resta L, Marsigliante S, Leo G, Fiorella R, Di Nicola V, Maiorano E. Molecular biopathology of metaplastic, dysplastic, and neoplastic laryngeal epithelium. Acta Otolaryngol Suppl. 1997 527:39-42.
• Inwang ER, Puddefoot JR, Brown CL, Goode AW, Marsigliante S, Ho MM, Payne JG, Vinson GP. Angiotensin II type 1 receptor expression in human breast tissues. Br J Cancer. 1997 75(9):1279-83. Erratum in: Br J Cancer 1997;76(6):827.
• Marsigliante S, Resta L, Muscella A, Vinson GP, Marzullo A, Storelli C. AT1 angiotensin II receptor subtype in the human larynx and squamous laryngeal carcinoma. Cancer Lett. 1996 Dec 20;110(1-2):19-27.
• Marsigliante S, Muscella A, Resta L, Storelli C. Human larynx expresses isoforms of the oestrogen receptor. Cancer Lett. 1996 Feb 6;99(2):191-196.
• Vilella S, Zonno V, Marsigliante S, Ingrosso L, Muscella A, Ho MM, Vinson GP, Storelli C. Angiotensin II stimulation of the basolateral located Na+/H+ antiporter in eel (Anguilla anguilla) enterocytes. J Mol Endocrinol. 1996 Feb;16(1):57-62.
• Marsigliante S, Muscella A, Vilella S, Nicolardi G, Ingrosso L, Ciardo V, Zonno V, Vinson GP, Ho MM, Storelli C. A monoclonal antibody to mammalian angiotensin II AT1 receptor recognizes one of the angiotensin II receptor isoforms expressed by the eel (Anguilla anguilla). J Mol Endocrinol. 1996 Feb;16(1):45-56.
• Marsigliante S, Muscella A, Ciardo V, Puddefoot JR, Leo G, Vinson GP, Storelli C. Multiple isoforms of the oestrogen receptor in endometrial cancer. J Mol Endocrinol. 1995 Jun;14(3):365-374.
• Marsigliante S, Mottaghi A, Muscella A, Ciardo V, Leo G, Storelli C. Effect of tumor size on the association between ps2 and cathepsin-d in primary breast-cancer. Int J Oncol. 1995 Jan;6(1):69-73.
• Resta L, Fiorella R, Marsigliante S, Leo G, Di Nicola V, Marzullo A, Botticella MA. The status of receptors in laryngeal carcinoma: a study of receptors for estrogen, progesterone, androgens and glucocorticoids. Acta Otorhinolaryngol Ital. 1994 Jul-Aug;14(4):385-392.
• Marsigliante S, Biscozzo L, Correale M, Paradiso A, Leo G, Abbate I, Dragone CD, Storelli C. Immunoradiometric detection of pS2 and total cathepsin D in primary breast cancer biopsies: their correlation with steroid receptors. Br J Cancer. 1994 Mar;69(3):550-554.
• Marsigliante S, Verri T, Barker S, Jimenez E, Vinson GP, Storelli C. Angiotensin II receptor subtypes in eel (Anguilla anguilla). J Mol Endocrinol. 1994 Feb;12(1):61-69.
• Marsigliante S, Leo G, D'Elia M, Vinson GP, Greco S, Puddefoot J, Storelli C. Relationships between tamoxifen binding proteins in primary breast cancer biopsies. Eur J Cancer. 1994 30A(11):1694-700.
• Marsigliante S, Biscozzo L, Resta L, Leo G, Mottaghi A, Maiorano E, Colucci G, Storelli C. Immunohistochemical and immunoradiometric evaluations of total cathepsin D in human larynx. Eur J Cancer B Oral Oncol. 1994 Jan;30B(1):51-55.
• Marsigliante S, Muscella A, Ciardo V, Barker S, Leo G, Baker V, Mottaghi A, Vinson GP, Storelli C. Enzyme-linked immunosorbent assay of HER-2/neu gene product (p185) in breast cancer: its correlation with sex steroid receptors, cathepsin D and histologic grades. Cancer Lett. 1993 Dec 20;75(3):195-206.
• Puddefoot JR, Baker VA, Bakkers B, Marsigliante S, Barker S, Panahy C, Goode AW, Carpenter R, Vinson GP. The nature and significance of multiple isoforms of the oestrogen receptor in breast tumours. J Mol Endocrinol. 1993 Aug;11(1):83-90.
• Barker S, Marchant W, Clark AJ, Jimenez E, Marsigliante S, Montiel M, Vinson GP. Comparison of COS cell transfected AT1A and AT1B angiotensin II receptors and angiotensin II receptor isoforms in rat tissues using isoelectric focusing. Biochem Biophys Res Commun. 1993 Apr 30;192(2):392-398.
• Marsigliante S, Resta L, Leo G, Mazzotta D, d'Amore R, Biscozzo L, Storelli C. Expression of cathepsin D in malignant and in the corresponding non-malignant node-negative laryngeal samples: correlation with receptors for androgen, glucocorticoid, oestrogen and progesterone. Cancer Lett. 1993 Feb;68(2-3):135-142.
• Marsigliante S, Leo G, Mottaghi A, Biscozzo L, Greco S, Storelli C. p53 associated with cathepsin D in primary breast cancer. Int J Clin Lab Res. 1993;23(2):102-108.
• Baker VA, Puddefoot JR, Marsigliante S, Barker S, Goode AW, Vinson GP. Oestrogen receptor isoforms, their distribution and relation to progesterone receptor levels in breast cancer samples. Br J Cancer. 1992 Dec;66(6):1083-1087.
• Marsigliante S, Greco S, Biscozzo L, Barker S, Baker V, Giuseppe L, Vinson GP, Storelli C. Transcriptionally active non-ligand binding oestrogen receptors in breast cancer. Cancer Lett. 1992 Oct 21;66(3):183-191.
• Marsigliante S, Biscozzo L, Barker S, Leo G, Puddefoot JR, Vinson GP, Storelli C. Type II oestrogen binding site is associated with the major 4S oestrogen receptor isoform in breast tumours. J Steroid Biochem Mol Biol. 1992 Aug;42(7):777-781.
• Marsigliante S, Leo G, Resta L, Storelli C. Steroid receptor status in malignant and non-malignant larynx. Ann Oncol. 1992 May;3(5):387-392.
• Marsigliante S, Biscozzo L, Greco S, Leo G, Storelli C. Relation of cathepsin D level to the estrogen receptor in human breast cancer. Int J Clin Lab Res. 1992 22(1):52-57.
• Marsigliante S, Baker VA, Puddefoot J, Barker S, Vinson GP. 4S oestrogen receptor isoforms and their distribution in breast cancer samples. J Mol Endocrinol. 1991 Dec;7(3):205-211.
• Marsigliante S, Puddefoot JR, Barker S, Goode AW, Vinson GP. Significance of the 8S complex in oestrogen receptor recognition. J Steroid Biochem Mol Biol. 1991 Nov;39(5A):703-711.
• Jimenez E, Marsigliante S, Barker S, Hinson JP, Vinson GP. Multiple forms of angiotensin II receptors in rat tissues. J Mol Endocrinol. 1991 Aug;7(1):21-26.
• Leo G, Cappiello G, Poltronieri P, Giardina C, Manca C, Storelli C, Marsigliante S. Tamoxifen binding sites heterogeneity in breast cancer: a comparative study with steroid hormone receptors. Eur J Cancer. 1991 27(4):452-6.
• Marsigliante S, Puddefoot JR, Barker S, Gledhill J, Vinson GP. Discrepancies between antibody (EIA) and saturation analysis of oestrogen receptor content in breast tumour samples. J Steroid Biochem Mol Biol. 1990 Dec 10;37(5):643-8.
• Marsigliante S, Puddefoot J, Vinson GP, Gilmore OJ. Effects of the presence of progesterone receptors on the relationship between enzyme immunoassay and steroid-binding assay for oestrogen receptors. Biochem Soc Trans. 1990 Aug;18(4):565-566.
• Vinson GP, Laird SM, Hinson JP, Mallick N, Marsigliante S, Teja R. Trypsin stimulation of aldosterone and 18-hydroxycorticosterone production by rat adrenal zona glomerulosa tissue is mediated by activation of protein kinase C. J Mol Endocrinol. 1990 Aug;5(1):85-93.